It is known that high nitrogen supply to fructan-accumulator plants induce a degradation of these storage carbohydrates to maintain high energy level to support growth and development (Westhafer et al., 1982). Tall fescue accumulates fructans in the shoot (Razmjoo et al., 1997; Lüscher et al., 2000) and these carbohydrates represent a good energy substrate and osmolytic compounds during the stress conditions, especially when photosynthesis decreases (Wiemken et al., 1995; Vijn and Smeekens, 1999). Mowing reduces leaf area and, as a consequence, tall fescue turf utilizes fructans especially when turf height becomes very low; however fructan reduction influences negatively the turf regrowth (Damiani et al., 2004). In this paper we have analyzed free sugars (glucose, fructose and sucrose) and fructans in tall fescue organs (leaf blade, shoot and root) from adult plants fed with different nitrogen concentration.